The experiment

Hmm...I have never thought about how to say this but I will just come out and say it.
In the beginning I didn't really know what I was doing, but I have set out a plan for what I have to do so I just go out and do it.
My thesis consists of four main parts of experiment. The first part is the Oxidation-reduction potential experiment. Basically this is just using a oxidation-reduction potential (ORP) meter to measure the ORP, which is measured in mV, of a liquid. This is an indirect measure of the ozone concentration of the liquid. The second part of the experiment is using Indigo reagent to measure the concentration of ozone in liquid. This is to dilute the Indigo solid into liquid and ozone will bleach the substance, so the original color is blue and the high ozone concentration will make the solution white or transparent. The third part of the experiment is to inhibit the bacteria in laboratory, using blood esculin plates. This experiment will include gas exposure, i.e. put bacteria on plates then expose to ozone gas/ozone liquid; liquid exposure, i.e. expose bacteria in liquid to ozone straight, or add ozonated liquid to liquid bacteria then grow bacteria on blood esculin plates; solid: grow bacteria on blood plate and when it is dry, apply ozone or apply ozone to liquid and put filter paper in the liquid, put this piece of paper on the dry blood plate that has bacteria. The last part of the experiment will involve cow work, but this part is unlikely to be done now.
To describe my work further, have to tell you more. Ozone gas is not good news to people's lungs so better put it into solution. However, ozonated solution has a lower bactericidal ability to ozone gas. The Indigo and ORP experiments aim to measure the concentration of ozone in liquid (water mainly). The bacteria experiment aims to find the best way to use ozone to kill bacteria. Mastitis is caused mainly by bacteria and that is why I am using ozone to kill the bacteria.
At this stage, ozone is still in the laboratory stage and it will take more than 10 years for it to be developed fully as a new teat sanitizer. Before then people will have to do more research on it, not necessarily me but also other people who is interested in this topic.